The crash came shortly after two other hijacked airlines were flown into the twin towers at the World Trade Center in New York.
The pictures, obtained by the legal rights group Judicial Watch, consist of frames taken every half second by a security camera at a Pentagon parking lot.
The video is now available on the group's Web site,according to Xinhua.
Judicial Watch said the video will dispel the so-called "conspiracy theory" which claims that the aircraft was shot down inflight, and that the Pentagon was struck by a missile Independent reports.
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A massive air strike with the use of cruise missiles and combat drones was launched on Kyiv on Friday, June 2 at night